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vsv g envelope expressing vector pmd2 g  (Addgene inc)


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    Addgene inc vsv g envelope expressing vector pmd2 g
    Vsv G Envelope Expressing Vector Pmd2 G, supplied by Addgene inc, used in various techniques. Bioz Stars score: 98/100, based on 14751 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/vsv+g+envelope+expressing+vector+pmd2+g/pMD2%2EG+(Plasmid+%2312259)/pm40412468-194-13-20
    Average 98 stars, based on 14751 article reviews
    vsv g envelope expressing vector pmd2 g - by Bioz Stars, 2026-09
    98/100 stars

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    Plasmid Preparation:

    Article Title: Unique properties of PTEN-L contribute to neuroprotection in response to ischemic-like stress
    Article Snippet: .. To produce LVPs human embryonic kidney cells were co-transfected with transfer vectors and second generation lentiviral packaging plasmid psPAX2 (Addgene plasmid #12260) and VSV-G envelope expressing vector pMD2.G (Addgene plasmid #12259; psPAX2 and pMD2.G were a gift from Didier Trono). .. Conditioned HEK cell medium was harvested 48 and 72 h after transfection, filtered with a 0.45 μm filter (Millex) and LVPs were precipitated with PEG-it virus precipitation solution (System Biosciences) overnight.

    Article Title: NOD2-induced IκBζ mediates a protective host response against epicutaneous Staphylococcus aureus infection.
    Article Snippet: .. Lentiviral particles were produced in HEK293T cells via lipofectamine 2000 transfection of the VSV-G envelope expressing vector pMD2.G (Cat. 12259, Addgene), a second-generation packaging plasmid psPAX2 (Cat. 12260, Addgene), and either an empty pLKO.1-puro plasmid as J urn al Pr e-p roo f 22 control (sh ctrl, Cat. 8453, Addgene) or pLKO.1-TRCN0000147551 encoding for shRNA against NFKBIZ (sh NFKBIZ). ..

    Expressing:

    Article Title: Unique properties of PTEN-L contribute to neuroprotection in response to ischemic-like stress
    Article Snippet: .. To produce LVPs human embryonic kidney cells were co-transfected with transfer vectors and second generation lentiviral packaging plasmid psPAX2 (Addgene plasmid #12260) and VSV-G envelope expressing vector pMD2.G (Addgene plasmid #12259; psPAX2 and pMD2.G were a gift from Didier Trono). .. Conditioned HEK cell medium was harvested 48 and 72 h after transfection, filtered with a 0.45 μm filter (Millex) and LVPs were precipitated with PEG-it virus precipitation solution (System Biosciences) overnight.

    Article Title: NOD2-induced IκBζ mediates a protective host response against epicutaneous Staphylococcus aureus infection.
    Article Snippet: .. Lentiviral particles were produced in HEK293T cells via lipofectamine 2000 transfection of the VSV-G envelope expressing vector pMD2.G (Cat. 12259, Addgene), a second-generation packaging plasmid psPAX2 (Cat. 12260, Addgene), and either an empty pLKO.1-puro plasmid as J urn al Pr e-p roo f 22 control (sh ctrl, Cat. 8453, Addgene) or pLKO.1-TRCN0000147551 encoding for shRNA against NFKBIZ (sh NFKBIZ). ..

    Produced:

    Article Title: NOD2-induced IκBζ mediates a protective host response against epicutaneous Staphylococcus aureus infection.
    Article Snippet: .. Lentiviral particles were produced in HEK293T cells via lipofectamine 2000 transfection of the VSV-G envelope expressing vector pMD2.G (Cat. 12259, Addgene), a second-generation packaging plasmid psPAX2 (Cat. 12260, Addgene), and either an empty pLKO.1-puro plasmid as J urn al Pr e-p roo f 22 control (sh ctrl, Cat. 8453, Addgene) or pLKO.1-TRCN0000147551 encoding for shRNA against NFKBIZ (sh NFKBIZ). ..

    Transfection:

    Article Title: NOD2-induced IκBζ mediates a protective host response against epicutaneous Staphylococcus aureus infection.
    Article Snippet: .. Lentiviral particles were produced in HEK293T cells via lipofectamine 2000 transfection of the VSV-G envelope expressing vector pMD2.G (Cat. 12259, Addgene), a second-generation packaging plasmid psPAX2 (Cat. 12260, Addgene), and either an empty pLKO.1-puro plasmid as J urn al Pr e-p roo f 22 control (sh ctrl, Cat. 8453, Addgene) or pLKO.1-TRCN0000147551 encoding for shRNA against NFKBIZ (sh NFKBIZ). ..

    Control:

    Article Title: NOD2-induced IκBζ mediates a protective host response against epicutaneous Staphylococcus aureus infection.
    Article Snippet: .. Lentiviral particles were produced in HEK293T cells via lipofectamine 2000 transfection of the VSV-G envelope expressing vector pMD2.G (Cat. 12259, Addgene), a second-generation packaging plasmid psPAX2 (Cat. 12260, Addgene), and either an empty pLKO.1-puro plasmid as J urn al Pr e-p roo f 22 control (sh ctrl, Cat. 8453, Addgene) or pLKO.1-TRCN0000147551 encoding for shRNA against NFKBIZ (sh NFKBIZ). ..

    shRNA:

    Article Title: NOD2-induced IκBζ mediates a protective host response against epicutaneous Staphylococcus aureus infection.
    Article Snippet: .. Lentiviral particles were produced in HEK293T cells via lipofectamine 2000 transfection of the VSV-G envelope expressing vector pMD2.G (Cat. 12259, Addgene), a second-generation packaging plasmid psPAX2 (Cat. 12260, Addgene), and either an empty pLKO.1-puro plasmid as J urn al Pr e-p roo f 22 control (sh ctrl, Cat. 8453, Addgene) or pLKO.1-TRCN0000147551 encoding for shRNA against NFKBIZ (sh NFKBIZ). ..



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    Nuclear localization of CasRx mediates optimal inhibition of HIV-1. Lenti-X TM cells were transfected with plasmid encoding CasRx-NLS-GFP, CasRx-NES-GFP, poly-gRNAs targeting HIV (polyHIV), or non-targeting control (polyNT). Twenty-four hours after transfections, the cells were infected with <t>VSV-G</t> pseudotyped HIV-iRFP. ( A ) Forty-eight hours after infection, expression of CasRx-GFP and HIV-iRFP were measured by flow cytometry; ( B ) %iRFP expressing cells in polyHIV vs. polyNT gRNAs transfected cells were compared in all experimental groups. The means ± s.e.m. are depicted as horizontal and vertical bars for each group, respectively. The Student’s t-test was used for statistical comparisons, and two-tailed p values are indicated.
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    Nuclear localization of CasRx mediates optimal inhibition of HIV-1. Lenti-X TM cells were transfected with plasmid encoding CasRx-NLS-GFP, CasRx-NES-GFP, poly-gRNAs targeting HIV (polyHIV), or non-targeting control (polyNT). Twenty-four hours after transfections, the cells were infected with VSV-G pseudotyped HIV-iRFP. ( A ) Forty-eight hours after infection, expression of CasRx-GFP and HIV-iRFP were measured by flow cytometry; ( B ) %iRFP expressing cells in polyHIV vs. polyNT gRNAs transfected cells were compared in all experimental groups. The means ± s.e.m. are depicted as horizontal and vertical bars for each group, respectively. The Student’s t-test was used for statistical comparisons, and two-tailed p values are indicated.

    Journal: Viruses

    Article Title: Efficient Inhibition of HIV Using CRISPR/Cas13d Nuclease System

    doi: 10.3390/v13091850

    Figure Lengend Snippet: Nuclear localization of CasRx mediates optimal inhibition of HIV-1. Lenti-X TM cells were transfected with plasmid encoding CasRx-NLS-GFP, CasRx-NES-GFP, poly-gRNAs targeting HIV (polyHIV), or non-targeting control (polyNT). Twenty-four hours after transfections, the cells were infected with VSV-G pseudotyped HIV-iRFP. ( A ) Forty-eight hours after infection, expression of CasRx-GFP and HIV-iRFP were measured by flow cytometry; ( B ) %iRFP expressing cells in polyHIV vs. polyNT gRNAs transfected cells were compared in all experimental groups. The means ± s.e.m. are depicted as horizontal and vertical bars for each group, respectively. The Student’s t-test was used for statistical comparisons, and two-tailed p values are indicated.

    Article Snippet: The CasRx, (pXR001; Addgene #109049), dCasRx (PXR002; Addgene #109050) plasmids, gRNA expression vectors (pXR003, pXR004; Addgene #109053 and #109054), Vesicular Stomatitis Virus glycoprotein (VSV-G) envelope expression vector (pMD2.G; Addgene #12259), lentiviral packaging plasmid (psPax2; Addgene # 12260), iRFP670 fluorescent reporter vector (piRFP670-N1; Addgene#79987) and pKLV2-U6gRNA5(BbsI)-PGKpuro2ABFP-W (Addgene #67974) were obtained from the Addgene repository (Watertown, MA, USA).

    Techniques: Inhibition, Transfection, Plasmid Preparation, Infection, Expressing, Flow Cytometry, Two Tailed Test